DNA/RNA Injection

DNA injection in the pronuclei of the zygote is the classical and most simple way to achieve transgenic mice, where the exogenous DNA or transgene is inserted randomly into the genome. In the CRISPR/Cas9 era, pronuclear injection is also widely used to introduce guide RNAs and template thet will induce genetic modifications in a very fast manner.

We inject both plasmid- and BAC-based transgenes, as well as RNA upon request.

We can design for you the constructs to be injected, or otherwise use your own reagents. In that case, you will provide us with the construct, amplified and digested (at least 210-20 micrograms). We will take over the last step of purification and quantification, prior to injecting it. We will inject 100-150 mouse zygotes, and transfer them to pseudopregnant recipient females. The pups generated are handed over to the user after weaning for genotyping and expression analysis.

By default, we generate founders with C57Bl/6N inbred genetic background, however other backgrounds can be considered upon request.

As this technique comprises the generation of a new genetically modified mouse line, the form includes information we need to gather in order to ask the Danish Authorities for the permission to generate this new line. Please note that you should ask for permission to maintain the line under your own license.

Please do not hesitate to contact us if you have any questions regarding this technique or the requisition form.