About the Core Facility – University of Copenhagen

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Core Facility for Transgenic Mice > About the Core Facility

About the Core Facility for Transgenic Mice

Genetically Modified Mice are important models in biomedical research

Analyses of defined mutations in specific genes are crucial to understanding the in vivo function of molecules expressed during development, in cell and tissue maintenance and repair processes, and in various diseases such as cancer, multiple sclerosis, or viral infection. Genetically modified mice are of tremendous importance in basic and clinical research and are the model of choice for investigating the molecular mechanisms underlying specific human diseases in an in vivo environment. Genetic alterations in mice can be used to establish new or improve existing mouse models. The use of such highly specific mouse models reduces time and the number of animals required to obtain disease-relevant results.

The Transgenic Mouse Core Facility supports the generation and archiving of genetically modified mice

At the Transgenic Mouse Core Facility scientists and expert technicians wlll help you establishing genetically modified mice using different techniques. We will discuss with you alternative strategies, generate and modify murine embryonic stem (ES) cells, inject mRNA, DNA, or ES cells into early embryos and transfer these embryos into pseudopregnant recipient mice. In addition we also “archive” genetically modified mice as embryos frozen in liquid nitrogen and rederive mouse strains from frozen stocks.

Equipment and mice

The TCF has special microscopes with micromanipulators for the injection of nucleic acids and ES cells into mouse embryos and equipment for freezing of mouse embryos. Embryonic stem cells are handled in state-of-the-art cell culture labs. At the animal house, we keep special mouse strains for the generation of mouse embryos and for the transplantation of modified embryos. All mice are kept in internally ventilated cages (IVCs) with enriched environment and are checked daily for their well-being.